Nikon Instruments

📣 We’re looking forward to the #ADLM2024 Clinical Lab Expo (formerly AACC)! Find us at booth 1010 starting Tuesday, 7/30 to see our latest products for the clinical lab, including the Ci-L plus upright microscope and PreciPoint O8 #SlideScanner. Click for more information: https://bit.ly/4deYRNY Association for Diagnostics & Laboratory Medicine #DigitalPathology #Microscopy #Imaging

Click chemistry has benefitted various bioimaging applications by providing a method for bioorthogonal labeling with high performance synthetic fluorophores and other probes, however it’s application in thick 3D samples has been hampered by a limited effective staining depth. Researchers now introduce Click3D, a method that combines a click labeling protocol optimized for staining depth and #TissueClearing – demonstrated for imaging of nascent RNA in a whole mouse kidney and other applications. Click to view the article: https://bit.ly/4fhoKOZ #Microscopy #Imaging #Fluorescence

Expansion #Microscopy #ExM has proven to be a popular method for #SuperResolution #Imaging, but existing protocols are generally long and intensive. Preprint research introduces BOOST, an ExM variant that uses microwave exposure to significantly accelerate key steps in the process, demonstrated for single-step 10X linear expansion of large millimeter-scale specimens in ~3 hours. Click to learn more: https://bit.ly/4ddvmMw

Let’s have fun talking #Tardigrades! Our #Microscopy #AppNote details imaging of GFP-expressing water bears using an AX / AX R #Confocal system. The transmitted detector makes it easy to simultaneously perform techniques such as brightfield and DIC. Click for more: https://bit.ly/45Uu5Yp

New research helps uncover how chemokines cause fast rolling lymphocytes in the vascular system to arrest – an important aspect of immune surveillance. The authors introduce biosensors for extracellular calcium and integrin activation and apply them to demonstrate that chemokine CCL25-triggered calcium influx reduces extracellular calcium levels to the point of allowing fast integrin activation and arrest. #LiveCell #Confocal #Microscopy for this study was performed using a Nikon A1 confocal system. Click to check out the article: https://lnkd.in/eUtjKe-C

Genetically encoded calcium indicators #GECIs are important for #LiveCell #Microscopy of calcium dynamics, but existing options generally aren’t well suited to detecting low concentrations. Researchers now introduce ultrahigh-affinity GECIs, exploiting a novel strategy for increasing affinity via linker length optimization in GECIs based on circularly permuted fluorescent proteins. This strategy should be generalizable to similarly designed indicators for glutamate and potassium. Click to view the article: https://lnkd.in/ehwqvhHY

Happy #AIAppreciationDay! From enhancing image resolution to automating complex analyses, #AI helps us provide researchers with powerful tools to unlock new scientific insights. Our AI-powered systems like the AX R and ECLIPSE Ji are designed to tackle the demanding imaging challenges with ease, driving precision and efficiency in the lab 🔬✔ Check out this article ⏬ #nikonmicroscopy #artificialintelligence #deeplearning #dl #machinelearning #ml #microscopy

DNA-PAINT labeling has revolutionized sample preparation for #SuperResolution techniques, especially single molecule localization #Microscopy #SMLM, but the background fluorescence from such probes can be problematic. New research introduces low background DNA-PAINT labels based on the self-quenching of fluorophore dimers when the imaging strand is in the unbound state. The authors demonstrate their utility for both SMLM and #STED. Click to view the article: https://bit.ly/3LnVSa3

🎉 We’re thrilled to announce that both our ECLIPSE Ji and ECLIPSE Ui microscopes have received an iF Design Award from iF DESIGN! The ECLIPSE Ji Smart Imaging System leverages #AI to support automated cell-based assays – learn more: https://bit.ly/3zJzuFM The ECLIPSE Ui Digital Imaging Microscope is designed for #DigitalPathology, setting a new standard for usability: https://bit.ly/469CBTG #NikonMicroscopy #Microscopy #Imaging #iFDesignAward2024

#Connectomics – the study of neural connections – has generally relied upon electron #Microscopy #EM to provide the necessary level of resolution for accurate reconstruction of connectomes. New preprint research combines #LightSheet, #SuperResolution expansion microscopy #ExM, and #DeepLearning-based segmentation for optical microscopy-based connectomics imaging with synapse-level resolution. Importantly, this approach allows for imaging of specific molecules. Click to learn more: https://bit.ly/4621oJ7 #Imaging #Neuroscience