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Chemical shift encoding-based water-fat magnetic resonance imaging (MRI) and placement of regions of interest (ROIs). (a) Representative proton density fat fraction (PDFF) map. (b) PDFF map with superimposition of manually segmented muscle compartments defined as ROIs: (1) right quadriceps muscle, (2) left quadriceps muscle, (3) right ischiocrural muscles, and (4) left ischiocrural muscles. The red lines around the thigh represent the segmentation of the entire thigh contour. Reused from [22] under Creative Commons license.